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Derek W Wright, Laura Mojsiejczuk, Afrida Mukaddas, Joseph Hughes, Robert J Gifford, David L Roberston, Daniel Goldhill, Edward C Hutchinson (MRC-University of Glasgow Centre for Virus Research, Sir Michael Stoker Building, Garscube Campus, 464 Bearsden Road, Glasgow G61 1QH Scotland (UK))
We present a novel web-based application for the interactive visualisation and analysis of influenza phylogenetic trees and identification of mammalian adaptations. This tool enables researchers to explore the evolutionary relationships among influenza strains while dynamically integrating user-provided sequence data. The application offers several key features: 1. Interactive phylogenetic tree visualisation with customisable metadata display, including H and N subtype information represented through colour-coded tree nodes. 2. Integration of user-uploaded query amino acid sequences, which are aligned against known sequences and visualised within phylogenetic context. 3. Identification and frequency analysis of known mammalian adaptation amino acid replacements in user-provided sequences. This powerful and user-friendly tool facilitates rapid exploration of influenza evolution, aids in strain characterisation, and supports the identification of potentially zoonotic variants. By combining phylogenetic analysis with dynamic sequence querying and adaptation marker detection, our application provides a comprehensive platform for influenza researchers and surveillance efforts.