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Pedro Machado, Jonathan Moffat, Joshua Lea, Louise Hughes (Oxford Instruments NanoAnalysis, High Wycombe, HP12 3SE, UK)
Multimodal imaging is the combination of several imaging techniques analysing the same specimen in an integrated or consecutive workflow. A significant challenge when doing multimodal imaging is the reimaging of the same region more than once with any chosen modality and the correlation and visualization of multiscale data. We have used multimodal imaging and correlative analysis to record changes in deer enamel and dentine structure before, during and after exposure to acidic solutions. The acquired data includes chemical, mechanical, and topographical analysis using Raman spectroscopy, electron microscopy (EM), Energy dispersive x-ray spectroscopy (EDS), and atomic force microscopy (AFM). We were able to characterise dynamic changes in the specimens by combining information from the different modalities in a correlative way to understand the effects of acid exposure on teeth.