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Getting poster data...
Elizaveta Loseva, Aniruddha Mitra, Erwin J.G. Peterman (Vrije Universiteit, Amsterdam, The Netherlands)
Single-particle imaging and tracking is a powerful technique for investigating molecular processes taking place in living cells. The acquired data contain temporally and spatially resolved information about separate molecules and can yield many valuable insights. However, its heterogeneity and complexity often makes analysis and interpretation of the results difficult. Our group works on deciphering mechanisms underlying functioning and maintenance of sensory cilia – chemical antennae of cells. This sensory organelle possesses specialized active transport system – intraflagellar transport (IFT), where large protein complexes, called IFT trains, powered by microtubule motor proteins, travel back and forth and bring the required cargo to the ciliary tip. We employ in vivo widefield fluorescence microscopy and a model organism C. elegans to obtain real-time information about position and dynamics of different proteins constituting the system. This poster is focused on exploring the dynamics of tubulin – the main component of the ciliary core structure, as well as on the ways to analyse, present and interpret the data we obtain.