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Michael Gray (Wellcome Trust Sanger Institute, Cambridge, England)
We believe our set of integrated software tools offers our annotators a genome visualization and annotation environment that cannot be matched in a browser application. The software's features support complex vertebrate annotation using a workflow which pulls together genomic data from around the world. Otter coordinates the workflow, and records and audits annotation edits. It performs on-the-fly alignments for verification of new and remapped evidence. Otter's filters can re-map data sources between different sequence assemblies. ZMap is a high-performance standalone genome browser/editor. Its vertical columns allow for side-by-side comparison of many tens of data sources. ZMap allows split panels to be scrolled in unison, and has a rich set of highlighting, searching and filtering options for coping with large-volume alignment and feature sources. Blixem is an interactive browser which provides display of features from ZMap in the context of one-to-many pairwise alignments at amino acid or nucleotide base level. Blixem is supported by Dotter for graphical dot-matrix comparison of sequences and by Belvu for viewing of multiple sequence alignments and phylogenetic trees.http://www.sanger.ac.uk/resources/software/anacodeannotools/