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Johannes Wollnik (Genomatix Software GmbH, Bayerstr. 85a, 80335 Munich, Germany)
The Genomatix Transcriptome Viewer provides a visual framework for integrating transcript and promoter annotations with RNA-seq and Bisulfte-seq data. In the standard view each transcript is drawn separately and in a novel splicing graph view identical exons and promoters are merged. Exons, promoters and splice junctions are drawn according to their read coverage. An integrated paired-end viewer shows the coverage of sequenced fragments and the distance of the respective paired-end reads along the transcript. Fragments are only considered if both mates uniquely map. This shows if the transcript is completely covered with reads and if they are within the expected distance. Another integral part is our gene fusion visualization, which allows users to interactively visualize both novel fusion products and known transcripts. Expression information is also displayed for each exon and splice-junction and can also be plotted for the entire fusion transcript at base resolution.