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Yangyang Bai, Matt Jones, Janielle Cuala, Lynne Cherchia, Lauro Sebastian Ojeda, Scott E. Fraser, Thai V. Truong (1219 W. 27th street)
Light field microscopy (LFM) records a 3D volume in a single snapshot. It provides game-changing improvements in imaging speed and throughput for a variety of biological imaging applications. In recent years, Fourier light field microscopy (FLFM) gain popularity with improved resolution and reconstruction speed by inserting a micro-lens array (MLA) at the Fourier plane of the optical path. In this work, we provide an implementation of FLFM platform that enables easy switching between wide range of imaging scenarios, from cell to system-level biology. Specifically, we construct the FLFM system with a wide field microscope body. By taking advantage of the integrated automatic noise-piece that carries multiple primary objectives in the microscope body, we achieve different imaging performance by switching to a different primary objective. Additionally, we also provide a MATLAB package for system designing the system parameters and data processing. We showcase the versatility of our system by recording of particle trafficking in the cell, calcium dynamics in mice islet and zebrafish brain neural activities.