Thumbnails:
List:
Year:
Category:
Session:
Poster:
Getting poster data...
Andrew McCluskey, Thomas D. Otto (University of Glasgow, Glasgow, UK; Université de Montpellier, Montpellier, France)
Bulk and single-cell RNA-sequencing (RNA-seq & scRNA-seq) has transformed our understanding for a plethora of organisms. Many studies have made use of both technologies, and the increased granularity of single-cell resolution has opened horizons to study organisms in unprecedented detail. However, the data generated by scRNA-seq is challenging to interpret and visualise. A common approach makes use of dimension reduction techniques such as Principal Component Analysis (PCA), and Uniform Manifold Approximation and Projection (UMAP). Other techniques are specialised for certain analyses, such as Potential of Heat-diffusion for Affinity-based Trajectory Embedding (PHATE) for investigating biological processes. In a typical analysis, these embeddings would be plotted in two dimensions, convenient for computer monitors, but sacrificing information which could be better shown in three dimensions.https://www.gla.ac.uk/schools/infectionimmunity/staff/thomasdanotto/