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Daniel Krentzel, Romain Laine, Matt Russell, Marie-Charlotte Domart, Ricardo Henriques, Lucy Collinson, Martin Jones (The Francis Crick Institute, London, UK; Imperial College London, UK; University College London, London, UK; Instituto Gulbenkian de Ciência, Oeiras, Portugal)
Correlative light and electron microscopy (CLEM) is a powerful imaging technique that enables visualisation of fluorescently labelled proteins within their ultrastructural context on a subcellular level. CLEM combines fluorescence microscopy (FM) and electron microscopy (EM). Each imaging technique requires a separate sample preparation workflow which introduces warping effects on a global and local scale between the resulting acquisitions. We have developed a graphical user interface (GUI) that automates the registration between FM and EM. Preliminary results of our algorithm are promising showing a significant reduction in alignment-time compared to manual registration going from various days to under three hours.