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Markus-Hermann Koch; Julian Uszkoreit, Stefan Loroch, Katja Kuhlmann, Martin Eisenacher, Hanna Diehl, Raphael Voges, Marco Oldiges, Helmut E. Meyer, Christian Stephan (MPC, Universitaetsstrasse 150 (ZKF I), 44801 Bochum, Germany)
Relative quantification of differing proteins requires accurate estimation of the percentage of molecules retained during the MS workflow from sample preparation to detection. This percentage we call a molecule's efficiency. This work presents the aspect of the label-free absolute quantification project Quasar that aims to predict such efficiencies. In detail we use a support vector regression approach where quantifyable peptide properties are mapped to the efficiency. As training data we apply efficiency estimates obtained from spiked-in proteins.