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J. Hornby*, B. van Rossum**, S. Thapa*, R. Metzler* and G. McGill*** (University of Potsdam Faculty of Science Karl-Liebknecht-Str. 24-25 14476 Potsdam OT Golm)
3D animation applications, predominately used by the entertainment industry, are increasingly accessible tools for visualizing and communicating complex scientific hypotheses. Many animations of molecular biology already feature protein models, derived by combining sequence and structural data. Yet there are few animations where the dynamics of molecular models are influenced by experimentally derived data. This work details the visualization of single molecule tracking (SMT) data generated through the application of total internal reflection illumination fluorescence microscopy (TIRFM), to track G Protein-Coupled Receptor (GPCR) membrane proteins across the surfaces of living cells. Principally, SMT data is imported into the 3D animation application, Autodesk Maya, which is then used to model the different modes of lateral cis-membrane GPCR diffusion. This results in a method for incorporating data from SMT directly into 3D animations of molecular biology. The secondary aim involved the categorization and selection of GPCR trajectories and looks to provide biophysical commentary on the utility of SMT data in visualizations of molecular diffusion.