Thumbnails:
List:
Year:
Category:
Session:
Poster:
Getting poster data...
Daniel Wendisch, Oliver Dietrich, Tommaso Mari, Saskia von Stillfried, et al. (see https://doi.org/10.1016/j.cell.2021.11.033)
Severe COVID-19 induces ‘acute respiratory distress syndrome’ (ARDS) in approximately 5% of patients. ARDS is characterized by diffuse alveolar damage and associated with prolonged respiratory failure and high mortality. Here, across 47 severe COVID-19 patients from two cohorts, we combined intravital imaging, physiological measurements, immunohistochemistry, electron imaging and single-cell genomics to investigate the mechanistic basis of lung injury. First, immunohistochemistry showed accumulation of CD163 macrophages in the lungs of diseased patients. These macrophages were furthermore shown to harbor SARS-CoV-2 RNA. Second, Single-cell RNA-seq of pulmonary immune cells from seven patients revealed the transcriptomic phenotype of these CD163 monocyte-derived macrophages which was distinct to infiltrating monocytes and tissue resident alveolar macrophages. Comparison of gene sets revealed a significant similarity between macrophage subsets found in COVID-19 and idiopathic pulmonary fibrosis (IPF). This association could be further supported through computational integration of multiple COVID-19 and IPF datasets by single-cell variational inference (scVI) over more than a tota