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S. Indino, E. Rosi, F.W. Baruffaldi Preis, F. Prignano, E. Donetti (via Luigi Mangiagalli, 31, Milan)
The identification of the specific cellular events underlying and/or triggering a diffuse chronic inflammatory skin disorder as atopic dermatitis (AD) is more and more challenging. Both cells of the adaptive immune system, i.e. Langerhans cells (LCs), and the predominant epidermal cell population, the keratinocytes, undergo pro-inflammatory milieu effects. In particular, the peculiar role played by interleukin (IL)-4 and IL-13 in promoting and supporting AD etiopathogenesis is still unclear. The aim of the present study is to dissect the early, specific, and direct effects of these pro-inflammatory cytokines on both keratinocytes and Langerhans cells in a 3D model of organotypic cultures of normal human ski, strictly mimicking the skin physiological condition. Bioptic skin fragments obtained after aesthetic surgery of healthy women (n=7) were cultured in a Transwell system with either IL-4 (50 ng/ml) or IL-13 (50 ng/ml) and harvested after 24 and 48 hours, with parallel control groups. All samples were processed for light microscopy (LM) and immunofluorescence (IF). As biomarkers of keratinocyte terminal differentiation, keratin (K)14 and K10, respectively expressed in the basal an