Thumbnails:
List:
Year:
Category:
Session:
Poster:
Getting poster data...
Ryan Lane (TU Delft, Delft, Netherlands)
As a standalone imaging method, electron microscopy (EM) suffers from two major limitations: slow rates of acquisition and the inability to natively provide targeted biological information. To overcome these limitations, we have integrated a fluorescence microscope together with an SEM. FM imaging facilitates ROI detection for successive high-resolution EM imaging. Registering datasets across different imaging modalities is typically a tedious and manual task, limited to small regions of interest. By implementing automated alignment routines during the acquisition phase and making use of open-source software for aligning images at scale, we are able to do hands-free, cross-modal registration over entire volumes. We demonstrate our correlative acquisition, reconstruction, and visualization workflow on thin sections of rat pancreas tissue.