Thumbnails:
List:
Year:
Category:
Session:
Poster:
Getting poster data...
Yibei Jiang, Raktim Mitra, Remo Rohs (1050 Childs Way, Los Angeles, CA 90089)
In the crowded genomic environment, transcription factors (TFs) navigate to its target DNA binding site and carry out the task of orchestrating gene expression. Many different TF families exist and more readout mechanisms are being discovered. The deconvolution of TF binding specificity has always been an important research area. However, no simple explanation can be given. This high specificity is not only due to the underlying sequence preferences of the core binding motif but also due to the structural elements on the protein surface, the DNA shape, as well as the surrounding genomic environment. Recently, in addition to the core motifs, the flanking sequences surrounding the core binding site are receiving increasing attention. Hox proteins, encoded by Hox genes, specify the segmentation of the fly embryo. Its core motif is a 4-5 base pairs long AT-rich sequence. Here, we investigate the binding preference of the HOX- Extradenticle (EXD) protein complex to genomic DNA using the data generated by high-throughput binding assay (SELEX-Seq) and molecular dynamics (MD) simulations to further probe the binding specificity of Hox Proteins, including the mechanistic influence of Arginihttps://www.cell.com/biophysj/pdfExtended/S0006-3495(23)04151-6