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Claudia Pommerenke, Tatiana Nedelko, Klaus Schughart (Helmholtz-Center for Infection Research, Infection Genetics, Braunschweig, Germany)Transcription analysis on a genomic scale via microarrays is a valuable tool to study different aspects of disturbances within a biological system. In order to investigate the role of the Mx1 resistance gene in the host response to influenza A infection, we examined the dynamic changes after infection of influenza virus A H1N1 during four days in the transcriptome from lungs of C57BL/6J mice deficient in Mx1 (Mx1-) and compared them to mice with intact Mx1 (Mx1+). By common functional analyses methods including PCA, clustering, GO analysis, GSEA, and SPIA provided by Bioconductor temporal differences in the innate immune response pathways and, more interestingly, in oxidative processes were identified. Furthermore, genes specifically upregulated on day 1 post infection in Mx1- mice but not in Mx1+, showed a delayed stimulation of one day in Mx1+. However, genes specifically high on day 1 post infection in Mx1+ but not in Mx1- mostly were not differentially expressed in Mx1- mice at later time points. The results indicate a protective role of oxidative stress responses triggered by Mx1 and specific candidate genes crucial for this protection in influenza A infected mice