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Thanos Theo and Tom Freeman (The Roslin Institute The University of Edinburgh Easter Bush Midlothian EH25 9RG Scotland, UK)Network analysis is playing an increasing role in our effort to understand the complexity of biological systems. This is primarily because of our ever increasing ability to generate large datasets, where the interaction or distance between biological components can be either measured experimentally or calculated. Accordingly, there is a need to visualize and analyze network graphs generated from these data. BioLayout Express3D has been specifically designed for visualization, clustering, exploration and analysis of very large network graphs in 2D and 3D space derived primarily, but not exclusively, from biological data. It supports the visualization, simulation and animation of graphs consisted of tens of thousands of nodes and of millions of edges. It achieves its results by utilizing the latest technology advancements in CPU and GPU hardware with the use of multithreaded parallelism and through the usage of the industry standards OpenGL and OpenCL APIs. BioLayout Express3D is a powerful tool in terms of the size of graphs that it can render and the ability of the user to explore and analyze these graphs in real-time and an elaborate, but still intuitive, user interface.