Getting poster data...
S Venkataraman, Bill Hill, Zsolt Husz, Ruben Schilling1, Alexander Schliep2, Lorna Richardson, Jeff Christiansen, Duncan Davidson & Richard Baldock (MRC Human Gentics Unit, Institute of Genetics & Molecular Medicine, Western General Hospital, Crewe Road South, Edinburgh EH4 2XU, UK)EMAGE is a visual database of gene expression patterns in mouse embryos. Gene expression data is spatially represented on and inside 3D digital reference models of mouse embryos at defined stages of development. Spatially annotating data into and onto these models to produce standardised representations is labour intensive and recently we have started to include full 3D imaged gene expression data. To this end, a number of manual spatial annotation approaches have been tried, while also (semi-)automated methods have been tested. We have also developed a method for delivering 3D data via web browsers which allows arbitrary slicing of volumes to show single virtual sections. We believe these methods will be an important step to understand spatial expression, especially for complex functional groups and to characterise gene expression spatially from several samples. 3D spatial mapping will become particularly useful once large-scale 3D datasets are acquired, where automated warping will ease the inclusion of such data into our reference embryos.