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Denise Schrama, Cláudia Raposo de Magalhães, Sofia Engrola, Pedro M. Rodrigues (Universidade do Algarve, Campus de Gambelas, Faro, Portugal)
High-quality proteins and protective omega-3 fatty acids are present in considerable amounts in fish. Unfortunately, allergies to fish affect 2-3% of the world’s population and might result in severe symptoms ranging from nausea and asthma to anaphylaxis. A calcium-binding, stable protein, known as β-parvalbumin (β-PV) is responsible for 95% of the allergic responses. The present study was conducted with the objective of decreasing the expression of β-PV in European seabass (Dicentrarchus labrax) to produce a low allergenic fish species. Comparative proteomics analysis was performed in fish muscle samples and polyacrylamide gels (2D-DIGE) were analysed using Samespots and subsequently with R software. Analysis of the identified proteins by MALDI-TOF/TOF, was performed on the STRING website generating protein networks with protein-protein interactions (PPI) which were further treated on Cytoscape. The KEGG pathway enrichment demonstrated the overrepresented pathways in the network. Proteomic software is very important for nutritional studies on fish from the aquaculture industry.