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Jeremy Henty, Gemma Barson (Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge)
Blixem displays gapped pairwise alignments of many match sequences against a genomic sequence. The genomic sequence is left intact, gaps inserted in the match are shown with "." characters, and an indicator between match characters shows where match sequences have extra hidden sequence residues not present in the genomic sequence. The match sequences may be DNA or proteins. DNA matches are shown in separate panels for the forward and reverse strands, or protein matches for the three reading frames of the strand being viewed. Blixem colour-codes the match quality and highlights features such as start and stop codons, splice sites and split codons. The choice of display elements is customisable, and can include SNPs, the intron/exon structure of transcripts, a coverage plot, species and gene names for each match, and unaligned sequence or polyA tails at the ends of matches. The top panel is an overview for rapid navigation, showing a cartoon of the location and quality of matches. The alignment text can be zoomed to balance legibility and extent. Buttons and keyboard shortcuts jump to the next boundary of the selected match so that splice junctions can be rapidly checked.