This hands-on tutorial will provide the participants with the tools to interpret macromolecular complexes and produce meaningful images and movies of the complex of interest using the software UCSF Chimera. You will be guided step by step trough the different aspects of this application from the loading of the molecules to the production of a movie in a way that will be understandable for total beginners and enjoyable for users with some experience. The course is intended for 10-20 participants and designed to be interactive, so any specific question proposed by the participant will be warmly welcome and some time will be dedicated to address specific issues.
The topics covered include:
• Loading of an EM map and basic manipulation of a structure (select atom/sequence/change size of the atoms etc.).
• Fitting a pdb structure in an electron density map, align pdbs.
• Transparency and color, how to use them to underline the biological significance.
• How to get a nice movie/ How to get your movie played everywhere (animated gif).
• Few command-line tips to make of you an “expert user”.
• Topic selected on the basis of students feedback.
The participants should bring your own laptops and have the latest stable release of Chimera and ImageJ installed prior to the tutorial. Handouts will be provided.
Tags: chimera, data visualization, tutorial, visualization, VIZBI, VIZBI 2014